Identification, by Electron Paramagnetic Resonance Spectroscopy, of Free Radicals Generated from Substrates by Peroxidase

Quantitative detection of plasma-generated free radicals in liquids by electron paramagnetic resonance spectroscopy

This work focused on qualitative and quantitative detection of oxygen free radicals in liquids after plasma treatment with an atmospheric pressure argon plasma jet by electron paramagnetic resonance spectroscopy (EPR). For the treatment, a shielded plasma jet, where the active effluent zone is surrounded by a protective gas curtain was used.

Electron paramagnetic resonance detection of free tyrosyl radical generated by myeloperoxidase, lactoperoxidase, and horseradish peroxidase.

Phagocytes secrete the heme protein myeloperoxidase, which is present and active in human atherosclerotic tissue. These cells also generate hydrogen peroxide (H2O2), thereby allowing myeloperoxidase to generate a range of oxidizing intermediates and stable end products. When this system acts on L-tyrosine in vitro, it forms o, o'-dityrosine, which is enriched in atherosclerotic lesions. Myelope...

Characterization of free radicals in soluble coffee by electron paramagnetic resonance spectroscopy.

EPR spectra of soluble coffee display single-line free radical signals in both the solid state and aqueous solution, along with signals from the paramagnetic ions Fe(III) and Mn(II). The intensity of the free radical signal in the pure solid was estimated to be ca. 7.5 x 10(16) unpaired electrons/g, and there was no significant change on dissolution in water. In aqueous solutions, however, the ...

Electron Paramagnetic Resonance Spectroscopy

Measurement of Free Radicals Using Electron Paramagnetic Resonance Spectroscopy During Open Aorto-Iliac Arterial Reconstruction

BACKGROUND Aortic cross-clamping during abdominal aortic aneurysm (AAA) open repair leads to development of ischemia-reperfusion injury. Electron paramagnetic resonance spectroscopy (EPR) spin-trapping is a valuable method of direct measurement of free radicals. The objective of the study was to evaluate the results of EPR as a direct method of free radical measurement and degree of inflammator...